Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure.
نویسندگان
چکیده
The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 was constructed by homology modeling using Archaeoglobus fulgidus esterase as a reference, and the thermostability-structure relationship was analyzed. Our results verified the predicted 3D structure of EstE1 and identified the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1.
منابع مشابه
Bioinformatics Analysis of Upstream Region and Protein Structure of Fungal Phytase Gene
Phytase increases the bioavailability of phytate phosphorus in seed-based animal feeds and reduces the phosphorus pollution of animal waste. Since most animal feeds for pellets are heated up to 65-80 °C, the production of a thermostable structure for phytase can be useful. In this study, we sought to perform bioinformatics analysis of the upstream region and protein structure of fungal phytase ...
متن کاملStructural basis for thermostability of beta-glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG102.
The three-dimensional structure of a thermostable beta-glycosidase (Gly(Tn)) from the thermophilic eubacterium Thermus nonproteolyticus HG102 was determined at a resolution of 2.4 A. The core of the structure adopts the (betaalpha)(8) barrel fold. The sequence alignments and the positions of the two Glu residues in the active center indicate that Gly(Tn) belongs to the glycosyl hydrolases of re...
متن کاملStress Analysis of the Human Ligamentous Lumber Spine-From Computer-Assisted Tomography to Finite Element Analysis
Detailed investigation on biomechanics of a complex structure such as the human lumbar spine requires the use of advanced computer-based technique for both the geometrical reconstruction and the stress analysis. In the present study, the computer-assisted tomography (CAT) and finite element method (FEM) are merged to perform detailed three dimensional nonlinear analysis of the human ligamentous...
متن کاملThe crystal structure of a hyperthermophilic archaeal TATA-box binding protein.
This study analyzes the three-dimensional structure of the TATA-box binding protein (TBP) from the hyperthermophilic archaea Pyrococcus woesei. The crystal structure of P. woesei TBP (PwTBP) was solved at 2.2 A by X-ray diffraction and as expected from sequence homology (36% to 41% identical to eukaryotic TBPs) its overall structure is very similar to eukaryotic TBPs. The thermal unfolding tran...
متن کاملSOLIDIFICATION MODELING AND DENDRITE STRUCTURE ANALYSIS IN A HORIZONTAL CONTINUOUSLY CAST BRASS BILLET
Abstract: The horizontal continuous casting process has received a significant attention for near net shape casting of non ferrous metals and alloys. Numerical Simulation has been widely used for process design and optimization of continuous casting process. In the present study, a 3-dimensional heat flow model was developed to simulate the heat transfer and solidification in a horizontal b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 72 4 شماره
صفحات -
تاریخ انتشار 2006